Unusual Modification of Bacteriophage Mu DNA
نویسندگان
چکیده
منابع مشابه
Genetic mapping of the inversion loop in bacteriophage Mu DNA.
An inversion loop seen in heteroduplex mapping of the DNA of mature Mu phage induced from a lysogen is observed also in defective lambda phage carrying one end of Mu. 14% of the DNA of Mu, including this region, designated the G loop, is shown to be to the right of all known genes in the prophage map. The inhomogeneous ends of Mu are observed as a separate phenomenon and appear in all mutants i...
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Excision of transposable genetic elements from host DNA is different from the classical prophage lambda type of excision in that it occurs at low frequency and is mostly imprecise; only a minority of excision events restores the wild-type host sequences. In bacteriophage Mu, a highly efficient transposon, imprecise excision is 10-100 times more frequent than precise excision. We have examined a...
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The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108...
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It is shown, by electron microscope observation of the structures of heteroduplexes, that Mu-1 DNA inserted into the bacterial episomes Flac and F(8)[1] is collinear with, rather than a circulation permutation of, the DNA of the mature Mu-1 bacteriophage. Observation of the position of the inserted Mu defines a point within the gene that has been inactivated (the lacI gene for Flac and a transf...
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UNLABELLED The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function. Recently, the CRISPR-Cas nuclease complexes were shown to mediate bacterial adaptive immunity by RNA-guided target recognition, raising the question...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.32.2.468-475.1979